VAMP4 protein (His tag) (80R-1178)
Purified recombinant Human VAMP4 protein
Overview
Overview
Synonyms | VAMP24 protein, VAMP4 protein protein, VAMP-4 protein, VAMP4, VAMP 4, Vesicle associated membrane protein 4 protein, isoform CRA a protein, VAMP 4 protein, isoform CRA b., VAMP 4 protein, Vesicle-associated membrane protein 4 VAMP 24 protein, VAMP-4, Vesicle associated membrane protein 4 protein, Vesicle associated membrane protein 4 protein |
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Species | Human |
Protein Type | Recombinant |
Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of VAMP4 protein (His tag) (80R-1178)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
Residues | 1-115 amino acids: MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH |
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Expression System | E.coli |
Purity | > 90% pure |
Molecular Weight | 14.5 kDa (123aa), confirmed by MALDI-TOF. |
Tag/Conjugate | His tag |
Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.1M NaCl, and 20% glycerol. |
Concentration | 0.5 mg/ml |
Storage & Safety
Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
Biological Significance | VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography. |
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