VAX2 antibody (10R-1276)

Mouse monoclonal VAX2 antibody

Synonyms Monoclonal VAX2 antibody, Anti-VAX2 antibody, Ventral anterior homeobox 2 antibody, VAX 2, VAX2, VAX 2 antibody, VAX-2 antibody, VAX-2, DRES93 antibody
Specificity Human VAX2
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen VAX2 antibody was raised in mouse using recombinant Human Ventral Anterior Homeobox 2 (Vax2)

Images

Immunofluorescent staining using VAX2 antibody (10R-1276)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-x100 in PBS. The cells were immunostained with anti-VAX2 antibody.

Specifications

Host Mouse
Clone VAX2A8F12
Isotype IgG1
Method of Purification VAX2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer VAX2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance VAX2 gene was named based on its high seqeunce similarity to VAX1 gene and is almost exclusively expressed in the ventral portion of the retina during development. It may play an important role in morphogenetic processes underlying the correct development of the eye, particularly in the establishment of a correct dorsoventral pattern.

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Images

  • Immunofluorescent staining using VAX2 antibody (10R-1276) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-x100 in PBS. The cells were immunostained with anti-VAX2 antibody.
  • Western Blot analysis of immunized recombinant protein using VAX2 antibody (10R-1276) | Left: VAX2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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