VE Cadherin antibody (10R-V102b)

Mouse monoclonal VE Cadherin antibody

Synonyms Monoclonal VE Cadherin antibody, Anti-VE Cadherin antibody, VE-Cadherin antibody, Vascular Endothelian antibody
Specificity Human
Applications FC, ICC, IHC-P, WB
Immunogen VE Cadherin antibody was raised in Mouse using HUVEC cells as the immunogen.
Assay Information IHC: Buffered formalin fixation is recommended with a fixation period of < 12 hours. High heat antigen retrieval in citrate buffer is suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells. FC: Use in the presence of Ca ++. PBS/2-5mM EDTA is recommended for cell detachment. WB: Non-reducing conditions may be needed, 2-5 mM Ca++ required in buffer.

Images

Immunocytochemistry staining using VE Cadherin antibody (10R-V102b)

Confocal fluorescent analysis of HUVEC cells using Anti-VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.

Specifications

Host Mouse
Clone BV6
Isotype IgG2a, kappa
Expression System 293 cells
Method of Purification VE Cadherin antibody was purified using Protein G affinity chromatography.
Form & Buffer Supplied in liquid form in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:500; IHC-P: 1:10-1:20; User optimization required for FC and WB.

Storage & Safety

Storage Stable for 1 year at 2-8 deg C from date of receipt.

General Information

Biological Significance Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell to cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Specificity determined by immunoprecipitation from a detergent extract of biotinylated cells. Antibody binding requires Ca++.

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Images

  • Immunocytochemistry staining using VE Cadherin antibody (10R-V102b) | Confocal fluorescent analysis of HUVEC cells using Anti-VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
  • Western blot analysis using VE Cadherin antibody (10R-V102b) | HUVEC cell lysate was probed with VE-cadherin antibody (1/2000 dilution).

Availability: In stock

Price: $495.00
Size: 100 ul
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