VEGF protein (His tag) (80R-1195)

Purified recombinant Mouse VEGF protein

Synonyms Vegf188 protein, Vegf-a protein, Vegf164 protein, Vegf120 protein, Vascular endothelial growth factor A isoform 3, Vpf protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) 5. Incubation time: 24 hr (after sample treatment) 6. Concentration range: 1 pg/ml ? 1 ug/ml 7. Detection method : BrdU assay

Images

Coomassie Blue stained SDS-PAGE of VEGF protein (His tag) (80R-1195)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 205-324 amino acids: MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
Expression System E.coli
Purity > 90% pure
Molecular Weight 16.3 kDa (141aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is 0.5-1.5 ng/ml. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance VEGF-A, also known as vascular endothelial growth factor, belongs to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. Recombinant VEGF-A protein was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of VEGF protein (His tag) (80R-1195) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $395.00
Size: 50 ug
OR
Shipping
View Our Distributors