VEGF121 protein (His tag) (80R-1082)

Purified recombinant Human VEGF121 protein

Synonyms VEGF121, VEGF-121, VPF protein, VEGF-A protein, Vascular permeability factor protein, VEGF protein, VEGF-121 protein, Vascular endothelial growth factor A isoform f protein, VEGF 121, VEGF 121 protein, VEGF A., Vascular endothelial growth factor A protein, VEGF121 protein, MGC70609 protein, Vascular endothelial growth factor A isoform f MVCD1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: NIH-3T3 (Mus mosculus/ embryo) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 24 hr (after sample treatment) Concentration Range: 100 pg/ml - 10 ug/ml Detection method: BrdU assay

Images

Coomassie Blue stained SDS-PAGE of VEGF121 protein (His tag) (80R-1082)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 207-327 amino acids: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
Expression System E.coli
Purity > 90% pure
Molecular Weight 16.3 kDa (142aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Liquid in 20mM Tris pH 8.0 containing 10% glycerol
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of VEGF121 protein (His tag) (80R-1082) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 50 ug
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