VEGF165 protein (His tag) (80R-2002)

Recombinant human VEGF165 protein (His tag)

Synonyms Vascular endothelial growth factor A isoform d protein, VPF protein, VEGF-165 protein, VEGF-A protein, VEGF165, VEGF protein, VEGF 165 protein, VEGF 165, VEGF-165
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of VEGF165 protein (His tag) (80R-2002)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 207-371 amino acids: MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Expression System E.coli
Purity > 90% pure
Molecular Weight 21.3 kDa (185aa), conformed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl(pH8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations VEGF165 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.
Endotoxin Levels < 1.0 EU per 1 microgram of protein (determined by LAL method)

General Information

Biological Significance Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of VEGF165 protein (His tag) (80R-2002) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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