Vimentin antibody (10R-2081)

Mouse monoclonal Vimentin antibody

Synonyms Monoclonal Vimentin antibody, Anti-Vimentin antibody, VIM antibody, Monoclonal Vimentin antibody, Anti-Vimentin antibody, VIM antibody
Cross Reactivity Human, Monkey
Applications ELISA, IHC, WB
Immunogen Vimentin antibody was raised in Mouse using a purified recombinant fragment of Vimentin(aa2-466) expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using Vimentin antibody (10R-2081)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin antibody with DAB staining.

Specifications

Host Mouse
Clone 4F2E9
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Vimentin is one of the five major groups of intermediate filaments, a distinct class of cytosekeltal proteins, occurring in mesenchymal cells. Localized vimentin can be detected in fibroblasts, endothelial cells, lymphoid tissues, and melanocytes.

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Images

  • Immunohistochemical staining using Vimentin antibody (10R-2081) | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin antibody with DAB staining.
  • Western Blot analysis using Vimentin antibody (10R-2081) | Western Blot showing Vimentin antibody used against Hela (1), COS (2), HEK293 (3) and U20S (4) cell lysate.

Availability: In stock

Price: $375.00
Size: 100 ul
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