Vimentin antibody (10R-V104a)

Mouse monoclonal Vimentin antibody

Synonyms Monoclonal Vimentin antibody, Anti-Vimentin antibody
Specificity Bovine vimentin
Cross Reactivity Amphibia, bovine, chicken, dog, human,monkey
Applications ELISA, IF, IHC-F, IHC-P, WB
Immunogen Vimentin antibody was raised in mouse using vimentin purified from bovine lens as the immunogen.

Specifications

Host Mouse
Clone VIM 3B4
Isotype IgG2a
Method of Purification Protein A affinity chromatography
Form & Buffer Supplied as a lyophilized powder. Reconstitute in 1 ml dist. water (final solution contains 0.09 % NaN3, 0.5% BSA in PBS buffer, pH 7.4)

Usage & Assay Information

Usage Recommendations IHC-F: 1:100, IHC-P: 1:100

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Vimentin is a member of the intermediate filament family of proteins that is especially found in connective tissue. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. This filament is used as a marker for mesodermally derived tissues, and as such can be used as an immunohistochemical marker for sarcomas.

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Availability: In stock

Price: $385.00
Size: 50 ug
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