Visfatin protein (His tag) (80R-1045)
Purified recombinant Human Visfatin protein
|Synonyms||VF protein, Nicotinamide phosphoribosyltransferase precursor protein, Visfatin protein, MGC117256 protein, Pre-B-cell colony-enhancing factor 1 protein, NAMPT protein, PBEF protein, PBEF1 protein, , pre-B-cell colony enhancing factor 1 protein, Nicotinamide phosphoribosyltransferase precursor protein, NAMPT protein|
Coomassie Blue stained SDS-PAGE of Visfatin protein (His tag) (80R-1045)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-491 amino acids: MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H|
|Purity||> 95% pure|
|Molecular Weight||57 kDa ( 511 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 8.0, containing 0.1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant human visfatin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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