VPS24 protein (His tag) (80R-1862)
Purified recombinant VPS24 protein
|Synonyms||VPS-24 protein, VPS 24 protein, VPS24, VPS 24, CHMP3 protein, VPS-24, NEDF protein, CGI-149 protein, FLJ38114 protein, Charged multivesicular body protein 3 protein|
Coomassie Blue stained SDS-PAGE of VPS24 protein (His tag) (80R-1862)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-222 amino acids: MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS|
|Purity||> 90% pure|
|Molecular Weight||27.2 kDa (242aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||VPS24, also known as Charged multivesicular body protein 3(CHMP3), belongs to the vacuolar sorting protein family and functions as a chromatin modifying protein. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Recombinant human VPS24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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