VPS28 protein (30R-1400)
Purified recombinant Human VPS28 protein
|Synonyms||VPS 28 protein, VPS-28, Vacuolar Protein Sorting 28 acuolar protein sorting 28 protein, VPS 28, VPS28, ESCRT-I complex subunit VPS28 protein, Vacuolar protein sorting 28 (yeast) protein, H-Vps28 protein, VPS-28 protein, Vacuolar protein sorting 28 homolog.|
Coomassie Blue stained SDS-PAGE of VPS28 protein (30R-1400)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-221 amino acids: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A|
|Purity||> 95% pure|
|Molecular Weight||25.4 kDa (221aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method )|
|Biological Significance||VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E. coli and purified by using conventional chromatography.|
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