VPS28 protein (30R-1400)

Purified recombinant Human VPS28 protein

Synonyms VPS 28 protein, VPS-28, Vacuolar Protein Sorting 28 acuolar protein sorting 28 protein, VPS 28, VPS28, ESCRT-I complex subunit VPS28 protein, Vacuolar protein sorting 28 (yeast) protein, H-Vps28 protein, VPS-28 protein, Vacuolar protein sorting 28 homolog.
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of VPS28 protein (30R-1400)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-221 amino acids: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
Expression System E.coli
Purity > 95% pure
Molecular Weight 25.4 kDa (221aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method )

General Information

Biological Significance VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of VPS28 protein (30R-1400) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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