VPS4B protein (His tag) (80R-3485)

Purified recombinant VPS4B protein (His tag)

Synonyms MIG1 protein, SKD1 protein, SKD1B protein, VPS4-2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie blue stained SDS-PAGE of VPS4B protein (His tag) (80R-3485)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-444 aa: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYLDRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSENESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVNEHDLLKLKKF TEDFGQEG
Expression System E.coli
Purity > 90% pure
Method of Purification VPS4B protein was purified by using conventional chromatography techniques
Molecular Weight 51.8 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Concentration 250 ug/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding.

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Images

  • Coomassie blue stained SDS-PAGE of VPS4B protein (His tag) (80R-3485) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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