VRK3 protein (His tag) (80R-2325)

Purified recombinant Human VRK3 Protein (His tag)

Synonyms Vaccinia related kinase 3 protein, VRK 3 protein, VRK3, VRK 3, VRK-3 protein, VRK-3, Inactive serine/threonine-protein kinase VRK3 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of VRK3 protein (His tag) (80R-2325)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLDAK DGRLFNEQNF FQRAAKPLQV NKWKKLYSTP LLAIPTCMGF GVHQDKYRFL VLPSLGRSLQ SALDVSPKHV LSERSVLQVA CRLLDALEFL HENEYVHGNV TAENIFVDPE DQSQVTLAGY GFAFRYCPSG KHVAYVEGSR SPHEGDLEFI SMDLHKGCGP SRRSDLQSLG YCMLKWLYGF LPWTNCLPNT EDIMKQKQKF VDKPGPFVGP CGHWIRPSET LQKYLKVVMA LTYEEKPPYA MLRNNLEALL QDLRVSPYDP IGLPMVP
Expression System E.coli
Purity > 85% pure
Molecular Weight 55.3 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Concentration 250 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance VRK3, also known as inactive serine/threonine-protein kinase, belongs to the protein kinase superfamily. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. VRK3 is widely expressed in human tissues and its protein localizes to the nucleus. Recombinant human VRK3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of VRK3 protein (His tag) (80R-2325) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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