WIF1 antibody (10R-2256)

Mouse monoclonal WIF1 antibody

Synonyms Monoclonal WIF1 antibody, Anti-WIF1 antibody, Wnt inhibitory factor 1 antibody, WIF1, WIF 1, WIF-1, WIF 1 antibody, WIF-1 antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen WIF1 antibody was raised in Mouse using a purified recombinant fragment of human WIF1 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using WIF1 antibody (10R-2256) (WIF1 antibody)

Immunofluorescence analysis of Hela cells using WIF1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Host Mouse
Clone 1G5
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance WIF1 inhibits WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation.

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Images

  • Immunofluorescent staining using Western Blot analysis using WIF1 antibody (10R-2256) (WIF1 antibody) | Immunofluorescence analysis of Hela cells using WIF1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western Blot analysis using WIF1 antibody (10R-2256) | Western Blot showing WIF1 antibody used against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
  • Immunohistochemical staining using WIF1 antibody (10R-2256) | Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 antibody with DAB staining.

Availability: In stock

Price: $345.00
Size: 100 ul
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