Wnt1 antibody (10R-1857)

Mouse monoclonal Wnt1 antibody

Synonyms Monoclonal Wnt1 antibody, Anti-Wnt1 antibody, Wnt-1 antibody, Wnt 1 antibody, INT-1 antibody, Wnt1, Wnt 1, Wnt-1, Wnt 1 antibody, Wnt-1 antibody
Cross Reactivity Human, Mouse
Applications ELISA, FC, IF, WB
Immunogen Wnt1 antibody was raised in Mouse using a purified recombinant fragment of Wnt1 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using Wnt1 antibody (10R-1857) (Wnt1 antibody)

FC analysis of Hela cells using WNT1 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 10C8
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin.

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Images

  • Flow Cytometric assay using Western Blot analysis using Wnt1 antibody (10R-1857) (Wnt1 antibody) | FC analysis of Hela cells using WNT1 antibody (green) and negative control (purple).
  • Western Blot analysis using Wnt1 antibody (10R-1857) | Western Blot showing WNT1 antibody used against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
  • Immunohistochemical staining using Wnt1 antibody (10R-1857) | Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 antibody
  • Immunofluorescent staining using Western Blot analysis using Wnt1 antibody (10R-1857) (Wnt1 antibody) | Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $345.00
Size: 100 ul
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