XBP1 antibody (10R-1263)

Mouse monoclonal XBP1 antibody

Synonyms Monoclonal XBP1 antibody, Anti-XBP1 antibody, XBP 1, Xbox binding protein 1 antibody, XBP2 antibody, XBP-1 antibody, TREB5 antibody, XBP1, XBP 1 antibody, XBP-1
Specificity Human XBP1
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen XBP1 antibody was raised in mouse using recombinant X-Box Binding Protein 1

Images

Western Blot analysis of immunized recombinant protein using XBP1 antibody (10R-1263)

Specifications

Host Mouse
Clone XBP1H6E5
Isotype IgG1
Method of Purification XBP1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer XBP1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis of immunized recombinant protein using XBP1 antibody (10R-1263)

Availability: In stock

Price: $190.00
Size: 50 ug
OR
Shipping
View Our Distributors