YARS2 protein (His tag) (80R-2294)
Purified recombinant Human YARS2 Protein (His tag)
|Synonyms||YARS 2 protein, YARS-2, YARS2, FLJ13995 protein, TYRRS protein, MLASA2 protein, YARS-2 protein, YARS 2, CGI-04 protein, MT-TYRRS protein|
Coomassie Blue stained SDS-PAGE of YARS2 protein (His tag) (80R-2294)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL|
|Purity||> 85% pure|
|Molecular Weight||53.7 kDa|
|Form & Buffer||Supplied in liquid form in 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA. Recombinant human YARS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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