YY1 antibody (10R-1610)

Mouse monoclonal YY1 antibody

Synonyms Monoclonal YY1 antibody, Anti-YY1 antibody, YY-1, Transcriptional repressor protein YY1 antibody, DELTA antibody, YY 1 antibody, UCRBP antibody, YY 1, YIN-YANG-1 antibody, NF-E1 antibody, YY-1 antibody, YY1
Specificity Human YY1
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen YY1 antibody was raised in mouse using recombinant Human Yy1 Transcription Factor (Yy1)

Images

Immunofluorescent staining using YY1 antibody (10R-1610)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-YY1 antibody.

Specifications

Host Mouse
Clone 3248C3a
Isotype IgG2b
Method of Purification YY1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer YY1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

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Images

  • Immunofluorescent staining using YY1 antibody (10R-1610) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-YY1 antibody.
  • Western Blot analysis of immunized recombinant protein using YY1 antibody (10R-1610)

Availability: In stock

Price: $395.00
Size: 50 ug
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