ZW10 antibody (10R-1465)

Mouse monoclonal ZW10 antibody

Synonyms Monoclonal ZW10 antibody, Anti-ZW10 antibody, ZW 10, KNTC1AP antibody, ZW-10 antibody, ZW-10, ZW 10 antibody, MGC149821 antibody, ZW10, HZW10 antibody
Specificity Human ZW10
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen ZW10 antibody was raised in mouse using recombinant Human Zw10, Kinetochore Associated, Homolog (Drosophila) (Zw10)

Images

Immunofluorescent staining using ZW10 antibody (10R-1465)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ZW10 antibody.

Specifications

Host Mouse
Clone 3363C4a
Isotype IgG1
Method of Purification ZW10 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ZW10 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.

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Images

  • Immunofluorescent staining using ZW10 antibody (10R-1465) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ZW10 antibody.
  • Western Blot analysis of immunized recombinant protein using ZW10 antibody (10R-1465)

Availability: In stock

Price: $190.00
Size: 50 ug
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